Dielectrophoretic Particle Characterization and Manipulation
نویسندگان
چکیده
This report discusses two aspects of the development of a high-throughput separation technique aimed at separating cells based on phenotypic differences in membrane composition using dielectrophoresis (DEP): automated particle characterization and electrodeless DEP separations. First we discuss an automated technique to measure cellular dielectrophoretic mobility as a function of frequency. Second, separation of a three-component suspension of polystyrene particles with diameters of 0.5 μm, 1.0 μm, and 2.0 μm was achieved using electrodeless DEP. An analysis of the geometry indicates that separations can be achieved spanning orders of magnitude in DEP mobility.
منابع مشابه
Combination Ultrasonic- Dielectrophoretic Particle Traps for Particle Trapping and Sample Purification in a Microfluidic Channel
Ultrasonic and dielectrophoretic particle manipulation have been studied for particle trajectory modification and particle trapping in microfluidic channels. We report an approach that combines dielectrophoresis (DEP) and ultrasonic fields to trap and concentrate particles and cells in an aqueous suspension. By simultaneously applying electric and ultrasonic fields to the sample, the favorable ...
متن کاملDielectrophoretic Characterization of Colloidal Particles Using Angled Microelectrode Arrays
This paper will present a characterization of a microfluidic device with embedded angled microelectrode arrays for the continuous flow-through dielectrophoretic separation of particles. The operation of the device is demonstrated using samples of colloidal latex spheres, achieving 100% particle deflection with low operating voltage of 10V. This characterization is essential for dielectrophoreti...
متن کاملDielectrophoretic effect of nonuniform electric fields on the protoplast cell
In recent years, dielectrophoresis based microfluidics systems have been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. In the current study the governing electric potential equations have been solved in the presence of cell for the purpose of ...
متن کاملFabrication of a Polymeric Microfluidic Device with Inkjet-Printed Silver Electrodes for Electrokinetic Bioparticle Characterization
We are developing an inkjet printing technique for patterning electrodes on polymeric substrates to create microfluidic devices for bioparticle applications. Electrode deposition is important for actuating electrokinetic phenomena in microfluidic devices, and polymeric substrates are becoming increasingly common. Dielectrophoretic (DEP) forces are induced via application of a spatially non-unif...
متن کاملHigh-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip.
Integrating different steps on a chip for cell manipulations and sample preparation is of foremost importance to fully take advantage of microfluidic possibilities, and therefore make tests faster, cheaper and more accurate. We demonstrated particle manipulation in an integrated microfluidic device by applying hydrodynamic, electroosmotic (EO), electrophoretic (EP), and dielectrophoretic (DEP) ...
متن کامل